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Latest known driver: 23.12.2020

Primer3 0.4.0 | Genuine & Reliable

Introduction

Do you need help optimizing these parameters for a specific target sequence you are working with?

GC Content: A range of 45% to 60% is ideal for stable binding without making the DNA "unzippable".

Backward Compatibility

Primer3 0.4.0 remains fully compatible with input files and command-line arguments from previous versions (0.3.x and earlier). No changes to the .primer3 file format are required.