Plant Tissue Culture Ppt Pdf Instant

Plant tissue culture (PTC) is the in vitro cultivation of plant cells, tissues, or organs in a sterile environment on a synthetic nutrient medium. This technique relies on totipotency, the biological principle that any single plant cell has the genetic potential to regenerate into a complete, functional plant. Core Concepts & Principles

• Slide 7: MS medium composition (table format)
• Slide 8: Auxins (2,4-D, IAA, NAA) – effects
• Slide 9: Cytokinins (BAP, Kinetin, Zeatin) – effects

1. Clear Learning Objectives: The first slide should state what the learner will know by the end.
2. History Timeline: A brief mention of Haberlandt (1902), Skoog & Miller (1957), and Murashige & Skoog (1962).
3. Laboratory Equipment List: Autoclave, Laminar Air Flow (LAF), growth room, pH meter, balance.
4. Media Composition: Macro & micronutrients (MS, B5, White’s), vitamins, carbon source (sucrose), gelling agent (agar), and PGRs (auxins vs. cytokinins).
5. Step-by-Step Protocol: Media prep → Sterilization → Inoculation → Incubation → Acclimatization.
6. Sterilization Techniques: For explants (NaOCl, HgCl2) and for equipment (autoclaving, UV, ethanol).
7. Growth Regulator Ratios: High auxin:cytokinin (callus/rooting) vs. high cytokinin:auxin (shoot proliferation).
8. Common Problems & Solutions: Browning (phenolics), contamination (bacterial/fungal), vitrification (hyperhydricity).
9. Real Images & Diagrams: Avoid clip art; use actual micrographs or lab photos.
10. References & Further Reading: Avoid plagiarism — good resources cite original papers (e.g., Murashige, T., & Skoog, F., 1962).

Case C: Synthetic Seeds (Synseeds)

• Process: Encapsulating somatic embryos in Sodium alginate.
• Use: Long-term storage and direct sowing.